Andy Moore

@aaandmoore.bsky.social

2839 Followers 1238 Following

User of microscopes. Interested in organelles and how they move. Husband, dad, intermediate filament apologist, and postdoc in the JLS lab at HHMI Janelia Research Campus.

Image processing monstrosity wordlessly beckoning you into its yawning maw

widefield movie of EB3-mNeonGreen in a cos7.

LysoTrackerGreen = orange, Keratin18=gray

MitoTracker Green (white), Spy555-tubulin, and SPY650-FastAct-X

Video of the endoplasmic reticulum labeled with mEmerald-Sec61B. The movie is a cumulative max intensity projection in a COS-7 cell revealing the complex organization of the ER and nuclear envelope.

Various movies of intermediate filament disassembly using the FilaBuster approach. These examples show local and global IF disassembly using HaloTag fusions with vimentin, a vimentin nanobody, or GFAP.

3D reconstruction of vimentin filaments (blue) and microtubules (light gray) in the perinuclear region (left) or flat cell periphery (right). The ventral plasma membrane is shown in gray.

Endogenously tagged vimentin-mEmerald (orange) and F-tractin-mCherry (actin filaments, gray) in a live COS-7 cell imaged with Airyscan microscopy.

Image of mouse embryonic fibroblasts with brightly colored vimentin intermediate filaments color coded based on orientation. white circles indicate region where the filament network has been focally disassembled.