Amir Mitchell @amitchell.bsky.social · Mar 14 We applied a pooled-genetic screening approach we developed to study how anti-cancer chemotherapies kill bacteria (previously published @eLife), this time around at a MUCH HIGHER throughput. These screens yielded ~ TWO million data points to analyze 🙀 (6/19)
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The idea was “straightforward”, grow all single-gene knockout (K/O) with each drug and find K/Os that are drug-sensitive/resistant to identify the killing mechanism (e.g., if K/Os of cell-wall synthesis genes are extra-sensitive, the drug likely targets the cell wall) (7/19)