Benjamin B Johnson

@benjaminbjohnson.bsky.social

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Early Career Postdoctoral Researcher at LUMC working with Christine Mummery and Richard Davis. 🔸 NWO-XS and Marie Curie Postdoctoral Fellowship grant winner 🔸 Stem Cell Disease Models 🔸 Myocardial Infarction and Cardiac Fibrosis #FluorescenceFriday

Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.

Cyan cellular extracellular matrix fibers are highlighted laying over the top of the orange hyaluronan secreted matrix after a scratch would assay.

Graphical abstract depicts automated setup to generate 3D cardiac mini tissues consisting of 3 cell types seeded into 384 well plates. The cardiac tissues are monitored using calcium traces to detect arrhythmias and subsequent rescue after compound addition.

Confocal microscopy shows a mini 3D heart (Cardioid) as a sphere full of wavy orange, reds, and cyan in the process of colliding and merging with a blood vessel organoid filled with deep reds and dark blues.