Chris Brooke

@christopherbrooke.bsky.social

1521 Followers 559 Following

Viral evolution, immunity, and infection biology. University of Minnesota. brookelab.org

Sunset view of the Mississippi river and beautiful downtown Minneapolis
IFN reporter assay results performed on supernatant collected form A549 cells treated with the indicated siRNAs and infected with IAV for 8 hrs.
Single cell RNAseq data from uninfected, untreated primary normal human bronchial epithelial cell cultures from 4 donors, differentiated at the air-liquid interface. Plots show the percentages of each cell type that express the indicated ISG.
Heat map showing correlation between expression levels in uninfected cells and inferred probability of expressing IFN after infection (top 20 hits shown).
Pseudotime reconstruction of A549 cells during IAV infection, showing movement of cells through time from pre-infection state (upper right) to two major post-infection states: IFN+ and IFN-
Single cell RNAseq data in which each dot represents the transcriptome of a single cell infected with A/Perth/16/2009, collected 16 hrs post-infection. Cells are colored red if they express detectable IFNL1, grey if they do not.