Coleen Murphy

@ctmurphy1.bsky.social

6475 Followers 5571 Following

Scientist, citizen, mom, and occasionally athletic

Mbappe after the match
Athena in her roast chicken pose
Athena sitting on the pizza that just arrived
A long-lost memory may resurface tonight
Athena in a tiny puzzle box
Athena enjoying life
TSA line stretches forever
A fox on snow
Nick Cave's work spanning the entrance
The Moon - crescent
I'm lifting my daughter up in front of a mountain lake
A carrot cake recipe - but with all the ingredients changed - and the user is criticizing the recipe
At Monument Square, honoring George Washington
The only one hired by Trump that is actually qualified for the position was Stormy Daniels
Princeton, NJ No Kings protest
Jane Boyer with annotation stickies throughout "How We Age"
Non-autonomous signaling may allow a longer timescale of memory function than neuronal memory regulation - that could be important in times of low nutrients - and is dependent on CREB transcription
The hypodermis is a highly metabolic tissue that transcriptionally resembles blood plasma and liver
Unlike young daf-2 mutants, ALL of older daf-2 mutants' memory extension is CREB-dependent
about half of daf-2's memory extension is gone when CREB is removed
CREB/crh-1 is broadly upregulated, including in "memory" neurons like AIM
lin-12 in the RIG neuron is required for hyp DAF-2 memory
osm-11 is a daf-2/daf-16 target expressed in the hypodermis that is upregulated in hyp DAF-2 but not neuronal DAF-2 degraded worms
Venn diagram of whole-worm targets of DAF-2 degraded in hypodermis vs neurons shows little overlap
DAF-2 degradation only in the hypodermis extends memory
Degradation of DAF-2 only in neurons extends memory, like daf-2 mutants
daf-2 mutants have longer memory than wild-type (N2)
We have three different pathogenic Pseudomonas species with three different small RNAs that have perfect 16-17nt matches to three different sequences in two genes (maco-1, vab-1) that act in the same genetic pathway to regulate daf-7 expression, ASI function, and the switch from attraction to avoidance.
PF15 training also induces avoidance of PA15, P. vranovensis, and the beneficial bacterium, P. mendocina, reinforcing the idea that these mechanisms overlap.
maco-1 expression is down in PF15-treated worms, as well as in the grandprogeny of Pfs1-treated mothers, suggesting that vab-1 somehow controls the expression of maco-1
maco-1 mutants/maco-1 RNAi - both naively avoid PF15
vab-1 RNAi induces avoidance of PF15
Pfs1 treatment alone induces avoidance in P0-F4
Predicted secondary structure of Pfs1 highlights 16nt perfect match to vab-1 in a stem loop
PF15 is fluorescent
Last known REAL Dire Wolf in the world
Dash and Athena on the bathroom floor