Doug Shepherd

@dpshepherd.bsky.social

615 Followers 416 Following

recovering alpinist and scientist.

Schematic on how to correct field curvature along light-sheet propagation axis.
Pseudo-colored fluorescence microscopy image of expanded paramecium and tetrahymena cells.
Adaptive optics oblique plane microscopy of 100 um thick mouse brain slice with immunofluorescence.
Immunofluorescence image of a mouse brain slice, with nuclei (magenta) and microglia (orange) shown.
California condor perched on a rock with the Grand Canyon in the background.
Left to right: pseudo-widefield, FISTA-SIM, Wiener filter SIM, and Bayesian SIM reconstructions of mitochondria labeled with PkMito Red in a live HeLa cell. Top to bottom: decreasing signal, starting at an average of 762 photons per pixel and dropping to an average of 16 photons per pixel.
An indoor bouldering route with a Twilight movie picture and quote.
View of the Cresenta valley and LA basin from the Middle Sam Merrill trail.
Photo of Grand Canyon from South Khabib trailhead.