GenomeTDCC

@genometdcc.org

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Technology Development Coordinating Center at NHGRI. Advancing innovation in genomic technology development through collaboration, promoting standards, and outreach in conjunction with The Jackson Laboratory 🧬💻🧪

Overview of NanoCortex Agentic System. https://www.biorxiv.org/content/10.64898/2026.05.19.726254v1/
Upcoming Webinar: Exploring How Genome Organization Shapes Biological Function Free registration: www.jax.org/genometechdev26 Join us for an exciting webinar bringing together leading researchers who are uncovering new layers of genome regulation and nuclear organization. Featured Speakers & Talks: Martha Bulyk – Revealing additional layers of cis‑regulatory codes governing transcription factor interactions Wenyi Feng – Linking cytoplasmic single‑stranded DNA detection to Fragile X Syndrome pathology Mitchell Guttman – RNA promotes the formation of spatial compartments throughout the nucleus This webinar will explore how regulatory DNA, RNA, and nuclear architecture intersect to shape biological function and disease mechanisms.
Genome Technology Development Webinar Series. Registration: www.jax.org/GenomeTechDev26 Session 1: Revealing Biological Complexity Through Spatial Profiling MARCH 26 @ 3:00 PM EDT Session 2: Charting Cellular Futures: Lineage Recording and Regulatory Network Insights MARCH 31 @ 2:00 PM EDT Session 3: Exploring How Genome Organization Shapes Biological Function APRIL 7 @ 1:00 PM EDT
The TDCC in conjunction with NHGRI and The Jackson Laboratory invite you to the free Genome Technologies Development Webinar Series. Registration: www.jax.org/GenomeTechDev26 Rapid advances in genomic analysis have increased the efficiency, accuracy, and depth of biological discovery, and these improvements are driven by innovative approaches in spatial profiling, single-cell genomics, functional genomics, and genome organization. This series brings together experts whose research continues to expand the frontiers of genome technology. The sessions are designed for postsecondary students, postdoctoral fellows, and biomedical researchers who are seeking a deeper understanding of how emerging tools are developed, applied, and refined. Each event includes scientific presentations and a roundtable discussion that examines current capabilities, ongoing challenges, and future opportunities in genome technology innovation. Revealing Biological Complexity Through Spatial Profiling 26, MARCH 3:00 – 4:00 PM ET Speakers: Fei Chen, Ph.D. Roy Wollman, Ph.D. Moderator: Ramalakshmi Ramasamy, Ph.D. Charting Cellular Futures: Lineage Recording and Regulatory Network Insights 31, MARCH 2:00 – 3:00 PM ET Speakers: Jay Shendure MD, Ph.D. Jason Buenrostro, Ph.D. Moderator: Yashwanth Ashok, Ph.D. Exploring How Genome Organization Shapes Biological Function 7, APRIL 1:00 – 2:00 PM ET Speakers: Martha Bulyk, Ph.D. Wenyi Feng, Ph.D. Mitch Guttman, Ph.D. Moderator: Inés Patop, Ph.D.
Exploring How Genome Organization Shapes Biological Function A webinar featuring Martha Bulyk, Wenyi Feng, and Mitchell Guttman. Registration: www.jax.org/genometechdev26 April 7, 1:00 PM - 2:00 PM ET
Free webinar featuring Jay Shendure and Jason Buenrostro. Charting Cellular Futures: Lineage Recording & Regulatory Network Insights. March 31 2pm ET. Register www.jax.org/GenomeTechDev26/
TDCC is hosting a webinar series featuring leading innovators. Free registration at www.jax.org/GenomeTechDev26
Revealing Biological Complexity Through Spatial Profiling webinar. Featuring Roy Wollman and Fei Chen. March 26 at 3 PM ET.
Images of the available talks on the genomeTDCC YouTube channel. These videos feature leading scientists who develop and use the latest genomic technologies.
Genomic Technologies Open Science Day on June 9. The event will be held at the Eric. P. Newman Education Center at Washington University in St. Louis and broadcast live online. Registration is free at https://www.jax.org/scienceday25/
Schematic of the fluorescence-based tethering assay as described in the recently published Nature paper by Joseph Lobel and Nick Ingolia, "Deciphering disordered regions controling mRNA decay in high-throughput".
From the BioRxiv pre-print article "Long-range massively parallel reporter assay reveals rules of distal enhancer-promoter interactions" by Yawei Wu et al. Fig. 1. Design of long-range MPRA. a, Design of the enhancer landing pad and the promoter landing pad. The enhancer landing pad contains a dual promoter driving expression of an mTagBFP fluorescent protein and a thymidine kinase (TK) gene for counterselection, flanked by FRT and F3 sites for RMCE. available under aCC-BY-NC 4.0 International license. was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made bioRxiv preprint doi: https://doi.org/10.1101/2025.04.21.649048; this version posted April 22, 2025. The copyright holder for this preprint (which The promoter landing pad expresses the mEmerald fluorescent protein and iCaspase9 for counterselection and is flanked by loxFAS and loxP sites for orthogonal RMCE. b-c, Workflow of long-range MPRA. eBC: enhancer barcode; rBC: random barcode; dBC: distance barcode. A barcoded enhancer library is integrated into pooled long-range landing pad cells through Flpase-facilitated recombination. After counterselection with Ganciclovir, cells with successful integration are enriched. Cells are sorted into different bins based on mScarlet fluorescence level. Genomic DNA is extracted from each bin; barcodes are amplified and sequenced. Reporter gene expression of each enhancer at each distance is calculated as the weighted average of fluorescence levels across bins.
Free event with developers and users of genomic technologies. Register at https://www.jax.org/scienceday25/ The objective of this forum is to provide post-secondary students, postdoctoral associates/fellows and biomedical researchers with a fundamental understanding of how these new technologies are utilized, as well as emerging opportunities and challenges associated with their optimization and use.
Single Cell Genomics Day hosted by Rahul Satija. Happening on April 25, 2025
The Genome TDCC YouTube channel has several videos on the latest genomic technology being developed by researchers supported by NHGRI. Highlights on new research publications and educational videos discussing new developments from nucleic acid synthesis, protein sequencing, large-scale genomic analysis and more.
A two-day virtual symposium featuring global experts in RNA mass spectrometry, sharing the latest research and innovations in RNA modifications and sequencing. Hosted by the NIH/NHGRI Center for Genomic Information Encoded by RNA Nucleotide Modifications
It's Pi Day! A time to honor the beauty of circles, the brilliance of 3.14159, and the joy of discovery. Let's celebrate with creativity, curiosity, and maybe even dessert.
Interpretive dance representing the new genome technology from the Shendure Lab, Genome-Shuffle-seq. Published in Science.
Breakdown of the funded categories and key terms within the Genome Technology program grant portfolio including Small Business Innovation Research grants (R43/R44) and R21, R01 grants across 18 small businesses and >40 different research institutes
2025 CRISPR training workshop
Center of Excellence in Genomic Science annual meeting
Breakdown of the NHGRI Genome Technology program grants and research areas that participate in the Technology Development Coordinating Center (TDCC).
The GenomeTDCC YouTube channel showcases videos from genomic technology developers, innovators, and expert users.
Tuesday (5/14) from 2:00 PM to 4:00 PM EDT, a panel of NIH small business program representatives will discuss SBIR and STTR funding. This webinar is hosted by XLerateHealth in collaboration with more than a dozen NIH Institutes and Centers, to learn more about how non-academic biomedical entrepreneurs can jump start their business and develop their technology through funding at the National Institutes of Health. This event isn’t just for non-academic entrepreneurs – Discussion will include opportunities for non-academic entrepreneurs and academic innovators to collaborate on these federally funded initiatives. Attendees will learn about SBIR/STTR and other non-dilutive funding opportunities and commercialization resources for NIH applicants and awardees, as well as XLerateHealth’s entrepreneurial-focused programs and resources to support your journey! Presentations will be followed by a moderated Q&A session.
Promotional poster of the Genome Technology Forum. Being held in-person and online on June 10, 2024 from 9:30 AM to 5:30 PM Eastern Time. Free registration at https://www.jax.org/gtf2024
DNA DAY 2024
Don't miss this amazing opportunity to hear from industry leaders and genome technology developers and users. They will focus on emerging technologies for understanding human genome functions discussing multi-omic analyses at a single-cell level, genome structure and function, and large-scale analysis of nucleic acid-protein interactions. Speakers will also cover advances in technologies for making and sequencing DNA and RNA. Sign up for the in-person experiential learning sessions with EpiCypher and Alida Biosciences to learn about epigenetics and epitranscriptomics. Keynote lecture will be delivered by esteemed speaker Stacey Gabriel (Broad Institute of MIT and Harvard). Stick around for the National Human Genome Research Institute (NHGRI) Small Business Program Expo with Alida Biosciences, BacStitch DNA, Claret Bioscience, DNA Script, Electronic Biosciences, EpiCypher, Flexomics, Primrose Bio, Ribotherapeutics, and more! https://www.jax.org/gtf2024
Advertisement for Single Cell Genomics Day live stream
A reporter system to assay RNA base editing activity of candidate RNA base editors (rBEs). a Reporter mRNA bearing twelve MS2 bacteriophage stemloops (yellowbars) downstreamof a Super folder GFP coding sequence (sfGFP) and a chimera bearing an MS2 coat-protein (MCP, black) domain fused to a candidate rBE (brown). b Strategy to test candidate rBEs. Plasmids encoding the constructs in a) are co-transfected into HEK293XT cells so that the MCP binds the MS2 stemloops in the reporter, and the rBE catalyzes RNA editing. After total RNA is isolated, targeted RNA sequencing is used to detect edits along the reporter sequence. (text from https://www.nature.com/articles/s41467-024-45009-4)
Development of tethered function assays for detecting direct induction of exon inclusion. a, Schematic of luciferase reporters used in the assays and resulting isoforms after cellular mRNA processing. b, Analysis workflow for calculating percent-spliced-in from luminescence measurements. c, Splicing gels of lucMAPT-30D splicing in response to co-transfection with MCP-fused positive and negative controls. d, Bar graph of lucMAPT-30D reporter readout as calculated from the workflow in b with the same conditions as c. e, Experimental workflow of tethering assays. The effects of recruiting 718 MCP-fused RBPs are tested in both reporter contexts.
Embryo-wide profiling of transcription, timing, and cell composition in zebrafish reveals processes that limit development robustness under increasing temperatures.
Structures of standard purine and pyrimidin nucleobases found in life and structures of mutually orthogonal synthetic xenonucleobases. Overview of construction of libraries and four nucleotide kmer models and observed current signals. Example traces overlaying observed mean signal (orange) with expected signal produced by XNA model (blue) or standard DNA model (gray) kmer model.
Workflow and cost analysis of the Bacterial Positioning System (BPS), and in vivo barcoding platform
Schematic of cis-based unfoldase approach on the MinION platform.
FLARE pipeline schematic
A snoRNA-tRNA modifcation network governs codon-biased cellular states. Published in PNAS