Johannes Hohlbein

@hohlbeinlab.bsky.social

2817 Followers 1342 Following

Single-molecule stuff, 🔬, 🦠 , 🧬, ☕, BTU, WUR, he/him, 🇩🇪->🇨🇿->🇩🇪->🇬🇧->🇳🇱+🇩🇪, Pubs: https://scholar.google.nl/citations?hl=en&user=WZZAydwAAAAJ&view_op=list_works&sortby=pubdate

Super-resolved image of DNA nanorulers.
Cat stretched along a wall.
Question on what is a DNA hydrogel and chatGPT showing a widget with a plot of f(x)=x function.
Custom microscopy build using a ASI Ramm scaffolding system.
Some Nikon microscopy objectives
Blue sky at dusk overlooking the marina of lake Senftenberg close to the university. At this time of the year still without boats.
Wings of three planes. One of them showing a cartoon mouse.
Picture of Halle (Saale) with a giant Christmas pyramid, the Market Church and the Red Tower. Somewhere hidden between Christmas market stands a statue of Händel can be seen.
Legendary old Trabant car from East Germany with extensive Christmas decoration.
Large mushroom. Maybe Imleria badia.
Snapshot from Google scholars showing 3333 citations.
Two sourdough loaves fresh from the oven.
Snapshot von ORCID.ORG welcher bestätigte Reviews auflistet.
Screenshot of an email newsletter wrongly saying that “power equals work times time”
Orchid.
Screenshot showing the software micromanager running. Center frame has white dots on black background indicating detected single fluorescent molecules.
Screen snapshot showing: (manuscript) received in December 2024, accepted in August 2025
Tomatoe plants witharig bit of ripening going on.
Two tomatoes plants showing many green (and some black) tomatoes but no red ones.
Small shelf showing the refrigerated milk section of a small Dutch supermarket. At least 25!different types, sizes, and brands of milk and milk-like drinks are visible.
Notre-Dame de Reims. Front view.
Two avocado plants in a pot. Height around 30cm each.
"Steel Jellyfish", 2006. Stainless steel, height: 4'7" (1.40 m). Location: Friday Harbor Labs, San Juan Island, WA, Julianva at the English Wikipedia, https://commons.wikimedia.org/wiki/File:Steel_Jellyfish_(GFP).jpg
"Excellent read!" quote on a post starting with "Good read."
Picture of woman reading. Tagline: “Sadly, I do my best proofreading after I hit sned“
Rock formation suitable for climbing in the Harz mountains.
10cm tall tomatoes plants in a pot of soil covered with straw
Picture of an adder.
"Horse Drawing Meme" annotated with lecture 1 (well drawn part of the horse) and lecture 10 (poorly drawn part of the horse)
Three airbus A380
Clock with hand drawn time. More info: https://mymodernmet.com/real-time-clock-maarten-baas/
Two arrows. One pointing to the right labelled with "my age". One pint to the bottom right labelled with "age of new students".
Sun dusk seen from within a building.
Two large fresh loaves of sourdough bread.
Four oven-fresh baguettes.
Tomato seedlings enjoying 29 deg centigrade in a mini green house.
Outline of a confocal microscope. Spectral properties of three different dichroic mirrors are shown. Students are asked to select the best matching one.
Super-resolution: *exists; Diffraction limit: meme picture "Am I a joke to you?"
Japanese restaurant. Food is on fire. 🔥
Voting letter.
Screenshot of the LinkedIn webpage showing that a number of my connections played today some games on LinkedIn...
Image of different DNA-targeting CRISPR/Cas systems: type I, II and V and there differences in Target Search, Target Recognition and Target Cleavage.
snapshot of settings in BlueSky muted words: Trump and Musk
RFT image analysis workflow. a) Simulated image with a sinusoidal wave pattern rotated by 130° (highlighted in orange) and a superimposed circular region with vertical lines: both patterns have the same wavelength. The green box indicates a sub-region (i). An inscribed box (blue, j) is then stepwise rotated around its centre. Here, we indicated the rotation angles of 40° and 130° by a magenta and orange vector, respectively. b) Line profiles for the blue box rotated for either of the two orientations. A flat or sinusoidal profile is obtained when summing the imaging intensity along the magenta or orange arrow, respectively. c) Fourier transform of profiles in b) shows the respective spectra. Integrating the spectrum in c) yields a scalar value, Vj,I, for each angle of rotation. d) Plotting the peak amplitudes over all rotation angles within the green subregion shows the expected peak amplitude around 130°. e) Summing all angular response curves in d) provides a peak amplitude profile for the whole image. Local maxima and dominant peaks are indicated by red dots or arrows, respectively. f) The global peak positions indicated in e) of the whole image can be used to bootstrap the fitting of frequency responses in sub-images (i.e., green box) seen in d) with several Gaussians. We can thus plot the dominant angle for each region g) or calculate the relative peak amplitude, for peak 1 in i) and peak 2 in j), for chosen angles within all sub-regions, thereby effectively mapping out the whole image.
View on the pier in Scheveningen (The Hague). Blue sky