James Manton

@jamesdmanton.bsky.social

1457 Followers 595 Following

Applied physicist & microscopist/microscopologist

A movie showing an acquisition through a cleared cerebellum, in which for every z plane four frames are acquired, each with a different combination of half-pixel shifts.
An panel of three images showing 1) raw data, 2) bicubic interpolated data on a two-fold finer grid, 3) properly upsampled data on a two-fold finer grid, for two parallel neuronal filaments that can only be resolved as separate entities in the upsampled image.
Screenshot of 'hardware' page of www.ctdvopm.org showing interactive CAD render and the start of the parts list.
A maximum intensity projection of a REPLICA-cleared kidney slice, immunostained for aquaporin 2 (cyan) and CD105 (magenta).
A gallery of cleared tissue images, including an iDISCO-cleared hairy chimpanzee skin organoid, a MACS-cleared mouse lung, an ECi-cleared mouse gut and an OptiMuS-prime-cleared mouse brain.
Plot of Strehl ratio as a function of depth, showing that a value greater than 0.8 is maintained for at least 18 mm for refractive indices in the range 1.33 to 1.56.
Zoomed CAD render showing chock for a refractive index of 1.45.
Illustrative schematic and CAD render of CtDvOPM system.
Maximum intensity projection of a mouse female reproductive tract labelled for PGP9.5.
Image of first two pages of manuscript.
A web warning from the National Cyber Security Centre, a part of GCHQ, stating: "This site may be associated with malicious activity or malware. Access to this site has been blocked by the Protective DNS Service Site: www.ctdvopm.org Please contact your local Network Administrator or IT support if you require further assistance"
A screenshot of the FOM talk planner, showing two talks on Sunday that occur at different times, and two talks on Tuesday that clash temporally.
A 3D maximum intensity projection rendering of a rotating mouse lung imaged on a light sheet microscope.
A calculation of the energy released by four-fold swelling of a 5 mm expansion microscopy hydrogel, containing 1 mmol of sodium acrylate. The calculation assumes a sphere of uniform charge density, in water, and gives an energy of 9.4E13 Joules, or approximately 22.5 kilotons of TNT.
A figure showing (a) point spread function measurements when imaging using a Sarstedt lumox dish, (b) representative images of NUP96 and NUP98 in HeLa cells, (c) the dishes themselves.
A comparison of imaging an expansion microscopy hydrogel with a water dipping objective lens, using either eye gel as an immersion medium or a combination of Immersol W, water and a thin FEP film. In both cases, the sample is supported by a thin FEP film rather than a coverglass.
A figure showing the lack of artefacts produced by our new algorithm, using a dataset of a 4-times expanded brain organoid imaged on a direct-view oblique plane microscope.
A figure showing that our approach works on a clathrin dataset with 4-fold undersampling, and correctly does not work on a 16-fold undersampled dataset.
A figure illustrating the principle of fully-sampled dataset reconstruction from apparently undersampled oblique plane microscopy data using deconvolution.
Refractometer measurement of a solution of ampyrone in water, for which n = 1.5184.
XY and XZ slices of a 4x-expanded cerebral organoid, showing both the 5 mm by 3 mm field-of-view and sub-cellular resolution.
Our new OPM system, in which the camera is placed directly after the secondary objective to directly detect the oblique plane.
An OPM system that redirects light from the secondary objective into the tertiary using a fibre transfer plate, as demonstrated by Hoffmann et al..
A diffractive OPM system that redirects light from the secondary objective into the tertiary using a transmissive diffraction grating, as demonstrated by Daetwyler et al..
A diffractive OPM system that redirects light from the secondary objective into the tertiary using a reflective diffraction grating, as demonstrated by Hoffmann et al..
An optical diagram of an oblique plane microscopy (OPM) system, featuring two back-to-back microscope systems, each comprising an objective and a tube lens, with a third tilted microscope system attempting to image a tilted plane. Because of the low numerical aperture, all the light leaving the secondary objective misses the third.
A photo of a Dell UP3218K 8K screen, showing a tiny window containing a full-chip Prime 95B image at 100 %.
Akaash Kumar, Jacob Lamb and Miguel Mestre kneeling in front of three 43" 4K displays, all set around a single user.
A flyer for the MRC Laboratory of Molecular Biology's PhD programme, with the application deadline being 3 December 2024.