Ján Bíňovský

@jbinovsky.bsky.social

1044 Followers 1261 Following

Incoming postdoc @ MPI of Biochemistry, Sokolova Lab 🇩🇪 | PhD @ CEITEC_Brno, Plevka Lab 🇨🇿 | biochemistry & structural biology 🧬🔬

The image is a mind map drawn on white paper, used for organizing thoughts before writing a paragraph. It contains various interconnected words and phrases, with many items circled, underlined, or checked off using green and red pens. Some words and phrases are marked with red circles, possibly indicating key points, while others have green checkmarks denoting completion. Additional notes and arrows are scattered across the sheet, suggesting a workflow for developing ideas. The lower section includes handwritten notes in red and green ink, outlining tasks like measuring angles, verifying structures, and calculating specific parameters.
Schematic illustration of phage attachment and penetration through a bacterial cell wall. The diagram shows multiple stages of a baseplate interacting with the bacterial envelope, progressing from initial attachment to full penetration. The bacterial layers, including peptidoglycan (PG) and cytoplasmic membrane (CM), are labeled, with viral components depicted in different colors, illustrating the stepwise mechanism of infection.
Conformational changes of the wedge modules, tail sheath initiator, and baseplate-proximal tail sheath proteins of phi812. The baseplate and baseplate-proximal tail of phi812 in native and contracted conformations are shown in cartoon representation. Conformational changes of the wedge module, tail sheath initiator, and baseplate-proximal tail sheath proteins are shown.
Cryo-electron micrograph of phage phi812 with a semi-contracted tail sheath. Phage is attached to a cell wall of S. aureus.
Panel A: Cryo-electron micrograph of the phi812 baseplate. The white dashed rectangle contains the coiled-coil, knob, and petal domains of the trimer of central spike proteins. Scale bar 100 â„«. Panel B: The surface representation of the cryo-EM reconstruction of the native phi812 baseplate is colored in gray except for central spike, hub, and weld proteins. The front part of the baseplate has been removed. Scale bar 100 â„«.
Conformational changes of the tripod complexes. The tripod protein complexes of phi812 in native and contracted conformations are shown in cartoon representations colored by subunits and by domain.
The baseplate and baseplate-proximal part of the tail of phi812 in native and contracted conformations are shown in cartoon representation. Conformational changes to a single baseplate arm are shown.
The baseplate core and wedge module proteins of phage phi812 are shown in cartoon representation and colored by protein type. The wedge module in the native conformation 1 is shown in lighter colors to emphasize two distinct conformations of the wedge modules in the native phi812 baseplate. The superposition of two neighboring wedge modules in native conformation 1 and native conformation 2 is shown. Protein names are indicated.
Cryo-electron microscopy image showing several bacteriophages attached to a bacterial cell surface. The scale bar indicates 200 nm, highlighting the nanoscale structure of the viruses.