Jeff Spence @jeffspence.github.io · May 30 And we considered a lot of different models of what dCas9-p300 actually does to chromatin tracks (How far away does p300 acetylate? How strongly would it increase signal in a ChIP-seq track? Does this interact with nucleosome occupancy? Do nucleosomes interfere with guide binding?)
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Overall, many of our models did a pretty job of ranking which guides would drive expression the most, but almost all of that performance came across genes. When trying to predict which guide would have the largest effect on the expression of a particular gene, our results were more mixed.