Jesse Engreitz

@jengreitz.bsky.social

1591 Followers 188 Following

Assistant Professor @ Stanford Genetics & BASE Initiative. Mapping the regulatory code of the human genome to understand heart development and disease. www.engreitzlab.org

scE2G schematic: scMultiome or scATAC-seq in healthy or diseased tissues, run through scE2G, produce enhancer-gene maps across cell types A/B/C including a risk variant.

Held-out CRISPR benchmark: n=189 positives, 4,175 tested pairs from 5 cell types. Weighted AUPRC bar chart with scE2G-Multiome and scE2G-ATAC highest, above Distance to TSS, ABC, and 8 other single-cell methods.

A Perturb-seq screen guided by species divergence uncovers pathways for collateral artery formation

1. Collect 1000s of CRISPR perturbations
2. Develop predictive models
3. Apply models to link risk variants to genes
4. Identify exceptions and iterate

bioRxiv - An unbiased survey of distal element-gene regulatory interactions with direct-capture targeted Perturb-seq

E2G Portal - PPIF genomic locus in IGV
https://e2g.stanford.edu/igv

E2G Portal - LD table for 1250566
https://e2g.stanford.edu

E2G Browser - rs1250566