Laura Markey

@lauramarkey.bsky.social

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barplot of individual leg skin microbiome profiles demonstrates diverse composition across subjects including multiple species of Cutibacterium, Corynebacterium and Staphylococcus. 4/8 female subjects have multiple vaginal microbiome species including Gardnerella and Prevotella but not Lactobacillus present at >10% abundance.
Left barplot shows that as mock community microbiome sample is diluted, qPCR and metagenomics continue to detect all taxa but 16S detects only Cutibacterium. Right barplot shows that for qPCR and metagenomics detect a diverse average microbiome from lower leg skin but 16S composition is dominated by Cutibacterium.
Left side of image shows a barplot of mock microbiome sample dilution series analyzed by qPCR, metagenomics and 16S sequencing- as the sample is diluted, qPCR and metagenomics continue to recall all taxa while 16S is increasingly dominated by Cutibacterium. Right side of image shows that similarly qPCR and metagenomics detect a diverse average leg skin microbiome while 16S is dominated by Cutibacterium.