Shutt Lab

@mitomorph.bsky.social

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Associate Professor at University of Calgary studying mitochondria.

Imaging of the Drosophila larval fat body. The mt-Kaede-HI-NESS reporter in the expressed from a UAS promoter driven by dau-Gal4. The Kaede mt-HI-NESS (green) is localized within the mitochondrial network (magenta). Nuclei are labelled with Hoechst (Cyan).
Live cell imaging of U2OS MFN2-KO or TFAM-Het lines known to have mtDNA release, which are transiently transfected with the mt-Kaede-HI-NESS reporter to visualize mtDNA nucleoids (green). Mitochondrial networks are labelled with Mitotracker DeepRed (purple). MtDNA nucleoids outside of the mitochondrial network are circled.
Live cell imaging of U2OS cells (WT or MFN2 KO) that are either stably transduced or transiently transfected with the mt-Kaede-HI-NESS reporter (green) to visualize mtDNA nucleoids. Mitochondrial networks are labelled with Mitotracker DeepRed (purple).