Osvaldo Burastero

@osvalb.bsky.social

860 Followers 949 Following

Biophysics enthusiast. Currently focused on data analysis: https://spc.embl-hamburg.de

Screenshot of CheMelt showing a popup asking the user which method to use to calculate errors.
Fitted curves provided by Hamborg et al., with CheMelt global model. Global slopes and global intercepts with exponential baselines
Unfolding dataset provided by Hamborg et al., 2020. The unfolding curves were monitored at 350 nm and 330 nm.
Abstract illustration showing several thermal unfolding curves at different denaturant concentrations
Simulated mass photometry results showing count distributions at a constant concentration of A (150 kDa) and seven different concentrations of B (30 kDa). B free is under the limit of detection (40 kDa). A and B interact with an equilibrium dissociation constant (Kd) of 1 nM.
espc poster details
Poster template done in Figma
Points: measured MST signal. Line: Fitted curved based on the estimated Kd of 1.3 micromolar.
Screenshot of ThermoAffinity showing that the Kd upper bound (for the fitting) is 5 micromolar.
Screenshot of ThermoAffinity showing that the selected units are 'Nanomolar', and that the hot region interval goes from 14 to 20 seconds.
An abstract representation of what happens during a circular dichroism measurement. Left- and right-circularly polarized light passes through the sample. One of the components is more absorbed.