Rob Fagan

@robfagan.bsky.social

2378 Followers 2412 Following

Lead for Molecular Microbiology @sheffielduni.bsky.social @molmicrosheffield.bsky.social #SEN parent, #AngelmanSyndrome Clostridial cell biology, AMR & bacteriophage structure and function He/Him https://sites.google.com/sheffield.ac.uk/fagan-lab/

Black and tan Cavalier King Charles, Toby, asleep on the wooden floor under our dining room table

Schematic showing a contractile bacteriophage binding to a bacterial cell envelope and contacting. The envelope is gram positive with an orange coloured S-layer on the surface

Flat tire on my car with a large hole in the sidewall

A large stack of exam papers awaiting marking.

Me and Johann Peltier standing on the side ofa canal in Leiden, the Netherlands. It's dark and the lights from bars are reflecting off the water

Yellow Labrador Pip lying on a blue vinyl floor in our vet's waiting room. She is pictured from above with her front paws in front of her. Her right paw is noticeable bigger than the left

Black and Tan cavalier king Charles spaniel Toby sleep in a brown leather armchair. Yellow Labrador Pip is asleep on the ground in the bay window behind the chair.

Mr4 and yellow Labrador Pip. They are walking side by side on a stoney bank with a shallow stream in the background

Figure 4 from https://doi.org/10.1099/mic.0.001665 - showing the use of genetic barcodes to distinguish strains in mixed populations during competitive growth in vitro or in the mouse gut

Figure 2 from https://doi.org/10.1099/mic.0.001665 - showingWestern blot detection of 3xHA and 6xHis tagged proteins, and purification of a StrepTagged protein

Figure 1 from https://doi.org/10.1099/mic.0.001665 - showing promoter activity from a panel of constitutive and inducible promoters in three Clostridia species

Yellow Labrador Pip asleep with her head on my shoulder. Just visible is the grey wet robe she's wearing

Mr4 sitting beside me on the couch. He's watching a video on his tablet and Labrador puppy Pip is asleep beside him

Pip wearing a very fetching grey bathrobe, asleep in my lap

A short video showing yellow Labrador puppy Pip running off a snow covered bank into a shallow river and playing in the water for a few seconds

Mr4 with his completed Lego technic truck

Mr4 leaning on a Lego manual, assembling a small truck transmission. In the background on the table is a partially built truck cab and a plate of grapes

Labrador puppy Pip wearing her very fetching green and orange harness, sitting on a green carpet in my office with some wooden drawers in the background. On her front right paw is a bandage covered with a green rubber boot

Dr Becky Menday cutting a three level sponge cake. On the table are many bottles of bubbly

Pip the Labrador and Toby the Cavalier King Charles spaniel lying side by side on our couch

A black and white photo of Mr4 and Pip the Labrador. They are laying on the sofa in opposite directions with Mr4's head resting on Pip's. Pip is asleep

A simplified version of Figure 4 from our paper https://doi.org/10.1371/journal.pbio.3002741 describing the evolution of vancomycin resistance in C. difficile. The shows phase contract and fluorescence microscopy images of a wild type and a vancomycin resistant C. difficile. In the fluorescence channel can be seen vancomycin -bodipy binding to the mid-cells of wild type cells but this labelling is absent in the resistant bacteria

Anne with her supervisors - William (Mack) Durham and me!

A drawing of yellow Labrador puppy Pip sitting on grass and with a purple giant microbe C. diff beside her

Pip the Labrador puppy on our couch. She has made a nest for herself among the cushions.

Yellow Labrador puppy Pip in the woods looking into a small stream flowing down a steep hill. The steam is actually artificial, the last remaining sign of a mill today used to stand on this site

Pip asleep on a brown couch

A shallow river running through a forest. A yellow Labrador puppy is just about visible, having just come out of the water

A close up photo of a very dirty Labrador puppy's face. She is a 7 month old yellow lab - although covered in mud so her coat looks darker

Mr4 pudding with his new Lego police car

Mr4, in a green and white horizontal stripey t-shirt, sitting at a low wooden table, looking at the instruction book for a Lego police car. The Lego pieces are spread out on the table in front of him

Selfie of Rob and Pip. Pip is a 7 month old yellow Labrador puppy

A view of the Eiffel Tower at night taken from a boat on the river Seine. The tower is brightly lit gold and a search light extends from the top

@lilia-elle.bsky.social, @funnirudh.bsky.social and Rob somewhere in Paris

@lilia-elle.bsky.social‬ in our PhD student office holding up her freshly printed poster - describing some of her work on the C. difficile stringent response

Selfie of Rob and Mr4 with the sea in the background and, about 100m away, a coastal town just visible

Pip the Labrador puppy sitting looking at me expectantly. She is sitting on a blue rug on a wooden floor.

Mr4 and Pip Labrador puppy sitting in the heather

Pip the Labrador puppy and Toby the cavalier king Charles spaniel asleep on the floor together

Labrador puppy Pip is asleep on her back with her nose resting on my left ankle. One front leg is resting on my left foot and under my right leg.

An Easyfig comparison of two DNA sequences showing that the sequence at the top has an additional three genes in the middle of the view that are missing from the bottom sequence. The regions either side are almost identical.

Pip the yellow Labrador puppy asleep on a grey carpet. She is wearing a green collar. Behind her is a brown leather armchair on which Toby the black and tan Cavalier King Charles is also asleep

Selfie of Rob sitting on the ground with a blue-grey couch in the background and an abstract painting on the wall. There is a small child's foot resting on his shoulder, resting against his cheek

Screenshot of the quoted post before also showing the first reply that reads "frog surveys aka tad polls"

A large stack of exam scripts on my dining room table waiting to be marked

A bottle of Yellow Spot whiskey

Pip the Labrador puppy posing for a photo in Padley Gorge. She is lying on leaf mulch among large broken rocks. On the background are trees on a downward slope onto the gorge

Labrador puppy Pip resting her head on my leg, looking at the camera. Also in my lap is a Mac laptop that I'm trying to work on

Photo of sleeping dogs. Black and tan Cavalier King Charles Toby asleep in a brown leather armchair. Yellow Labrador puppy Pip is asleep under the arm chair, with her back legs just in a fluffy bed visible on the background

A David Mellor chef's knife in a presentation box. The blade is Sheffield steel, set in a dark wood handle

Labrador puppy Pip asleep in a fluffy dog bed. Her head is hanging off the side of the bed and is resting on the grey carpet

Yellow Labrador puppy, Pip, asleep in a fluffy dog bed

Two Labrador puppies play fighting

Mr3 watching two Labrador puppies play fighting as a third walks behind him

Toby and Pip asleep near each other on the floor

Pip, the Labrador pup, asleep in a fluffy tan bed on the floor alongside Toby, the Cavalier King Charles, asleep in a brown leather armchair with his chin on the arm

A photo of our living room. On the right, in the foreground, yellow Labrador puppy Pip is sitting on a couch. It's a brown fabric couch but covered with a navy throw sand with some tan cushions visible. On the background Cavalier King Charles Toby is sitting on a brown leather armchair in a bay window. There are white horizontal shutters on the window. To the left of a fluffy dog bed on the floor and the corner of a TV on a wooden cabinet

The empty stage of the Sheffield City Hall main hall. The stage is lit and there's a single microphone and a small table with a bottle of beer. A large screen at the back of the sausage reads "Greg Davies Full Fat Legend"