Hans Pfalzgraf

@scihans.bsky.social

1124 Followers 374 Following

Research Scientist at ProImmune. Biochemistry to help make Ankyron® protein binders. Former crystallographer. I love #SciComm, #Sustainability, #Climbing and #EDM. Views are my own. He/Him.

Adenosine molecule represented as balls and sticks with carbon in red, oxygen in blue and nitrogen in green 🤢
PC screen of images at different focus heights of a crystallisation drop with crystals. Pan to the imager where an illuminated crystallisation tray is moved under the camera.
Smiling Hans in a lab coat sitting with a crystal-harvesting wand in front of a Crystal Shifter robot with a microscope and a foam bucket of liquid nitrogen.
A smiling Hans with a staff lanyard, standing inside the experimental hall of Diamond Light Source, the UK national synchrotron. There are the roofs of a couple beamlines and a yellow crane at the back. The curved wall has zone 4 indicated in red.
A pretty protein structure made mostly of beta-sheets. There are two hemes, one per monomer. In P460, the heme is linked to a lysine residue and can do hydroxylamine oxidation. In one c prime beta, the heme has peroxidase-like activity. In another,it does gas binding.
Line-angle formula of a heme c with a double crosslink to a lysine residue. The modification compared to a single N-C crosslink are highlighted in red: a double bond is added between the carbons CD and CE of the lysine. The double bond of pyrrole ring D has shifted away from the new C-C crosslink to become exocyclic.
Smiley Hans in the lab wearing proper PPE. Using a multichannel pipette to dispense buffers into a crystallisation plate based on electronic lab notes on a tablet.
A noisy red silhouette of a cat with a half-faded tail. This happens when you take the phases of a tail-less cat and the Fourier magnitudes of a cat with tail.
Circular graph with peaks appearing when switching between room temperature and cryo structures. They flag lower B-factors.
A few small tubes with dark green liquid, arranged in a half-circle
Rack of labelled tubes containing red (CoCl2), blue (CuSO4), green (NiCl2), orange (FeCl3) and colourless solutions (McCl2 and ZnSO4)