Stephan Wagner

@stephanwagner.bsky.social

256 Followers 279 Following

Lecturer for microscopy 🔬 @UniBonn with key interests in biosensors and redox biology. 🌱 scientist. 🚲 lover.

Scheme how genetically encoded biosensors can shine light on plant metabolism. Intrinsic sensors bind their analyte directly, extrensic sensors require a sensing domain fused to one, or more, fluorescent proteins. In paraplexing experiments, multiple sensors are individually expressed in plants and their responses are recorded in parallel. In multiplexing experiments, two or more sensors with non-overlapping excitation and emission spectra are expressed in the same plant. Sensor responses, either recorded in paraplexing or multiplexing experiments over time and through appropriate instruments, such as fluorescence microscopes or plate readers, can be used to create increasingly complex metabolic networks.