Zach Stevenson

@zachcst.bsky.social

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Postdoc, Shendure lab University of Washington/HHMI. Synthetic circuit development. Ph.D, Phillips Lab University of Oregon. Genetic engineering and experimental evolution. Zachstevenson.com

Plot showing ivermectin molarity on the X axis and percentage of the adult on the Y after 72 hours of growth in liquid culture. We see the wild type strain is significantly decreased in development as the ivermectin concentration increases. While the mutant strain is relatively the same.

Plot showing concentration of ivermectin on the X axis and selection coefficient on the Y. These are for the mutant strain. Wildtype is normalized to zero and is represented by a blue dotted line. We see the mutant strain increases it's selection coefficient as the concentration increases.

Six stacked plots where we see wildtype worms competing vs mutant worms. We see that the mutant worms are loosing till around 3nM concentration of ivermectin.

Schematic overview showing strains of C. elegans receiving barcodes by TRADIS. Then mixing these populations to compete them by serial transfer.