Zid Lab

@zidlab.bsky.social

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UC San Diego Biochemistry studying RNA localization and mitochondria

Violin plots of the cytosolic and membrane stability of cytosol localized, membrane localized, all mitochondrial, and mitochondrially localized mRNAs.
Model of eIF5A depletion leading to ribosome stalling and a mitochondrial import stress response.
eIF5A depletion in the tif51A-1 temp sensitive strain causes increased mitoCPR RNA expression and mislocalization of mitochondrial proteins to cytosolic storage depots called MitoStores.
TIM50 mRNAs in green are highly localized with the mitochondria marked in red. https://doi.org/10.7554/eLife.57814
Ribosomes start to build up ~300bps upstream of a stretch of 7 proline residues and then do not seem to make it through the ribosome stall.