Maitreyi Das

@daslabpombe.com

2327 Followers 1665 Following

We study cell polarity and cytokinesis in the fission yeast model system with emphasis on GTPase signaling patterns @BostonCollege. Immigrant. She/her. www.daslabpombe.com

Spn3-GFP localized to the mitochondria, and Rga4-mCherry appears diffuse along the cortex.
Montage of time-lapse of Spn3-GFP and Sad1-mCherry expressing cells during mitosis. Red asterisk marks the onset of mitosis. Red boxes highlight decreasing Spn3-GFP filament intensity along the cell side throughout mitosis. Sad1-mCherry labels the spindle pole body and indicates mitosic progression.
Spn3 and Spn1 colocalize to short filaments along the long axis of interphase cells.
Cells lacking Spn1 and 4 but not Spn2 and 3 show high septation index, indicating cytokinesis defects. But deletion of each of the 4 septins show high monopolarity index, indicating a polarity defect.
G. Montage of Rga4-GFP in spn1+ cells during interphase, during mitosis, and in spn1Δ cells during interphase, taken over a 5-minute time-lapse. Red arrowhead marks an Rga4-GFP puncta at the start of the time-lapse. only interphase spn1+ cells show immobile Rga4-GFP puncta along the cell sides. H. Montage of Rga4-GFP recovery after photobleaching during interphase and mitosis in spn1+ and spn1Δ cells taken in 15-second intervals. Red circles indicate the photobleached region of interest (ROI). Red lightning bolt indicates the time point of photobleaching. Rga4-GFP recovers after photo bleaching in spn1Δ regardless of cell cycle phase but not in spn1+ interphase cells. I. FRAP recovery rates of Rga4-GFP signal intensity within the ROI over time in spn1+ and spn1Δ cells during interphase and mitosis. J. T1/2 fluorescence recovery times of listed conditions.
Middle plane images of Rga4-GFP in spn1+ and spn1Δ cells. Red brackets highlight Rga4 distribution along cell sides in interphase cells. Red asterisks highlight dividing cells. Rga4-GFP in interphase cells in spn1Δ appears diffuse and also at teh cell ends.
Dinner table with mutton pulao, karahi chicken and raita.
Atb2-mCherry labeled spindle microtubule dynamics in DMSO or CK666-treated synchronized cdc25-22 cells post cell cycle release. Severe bending of microtubules is seen in CK666-treated cells.
Fim1-GFP localizes adjacent to Ppc89-mCherry 2 minutes prior to onset of mitosis
Loss of branched actin after preprophase arrest does not block mitosis when released. Mitotic progression in nda3-KM311 cells after release from preprophase arrest in DMSO or CK666-treated cells. Quantification of the percentage of cells that progress to mitosis at respective time intervals after the release from prophase arrest.
Representative images display accumulation of NLS GFP in the nucleus in DMSO or CK666 treated cut12.1 NLS-GFP strain grown at 36°C for 4 and 5 hours. CK666 treated cells show higher levels of NLS-GFP.
Redistribution of Sad1-mCherry and Cut11-GFP in synchronized cdc25-22 released into the cell cycle for 30 mins after DMSO or CK666 treatment. CK666 treated failed to form Sad1 and Cut11 rings.
Plo1-GFP localization in DMSO or CK666-treated synchronous cdc25-22 cells released into the cell cycle. Plo1-GFP localization at the SPB is failed in CK666 phenotype 1, delayed in phenotype 2 and recruited earlier but the mitotic onset is in phenotype 3.
DMSO or CK666 treated synchronized cdc25-22 fimΔ cells 30 and 60 minutes after release from cell cycle arrest. bqt4-mCherry labels NE and atb2-mCherry labels microtubules. Red arrowhead shows monopolar spindles. Fimbrin deletion cells show mitotic defects regardless of CK666 treatment.
Branched actin inhibition blocks or delays mitotic entry. Time-lapse microscopy of DMSO or CK666-treated cdc25-22 synchronized cells released from cell-cycle arrest. Cut11-GFP marks SPB.
Protein-Protein interaction analysis of the downregulated gene in the G1/S phase of cdc10-129 cells using string analysis
Protein-Protein interaction analysis using string for upregulated genes in the G1/S phase of cdc10-129 cells using string
Experimental design for high-throughput mRNA sequencing. cdc10-129 mutants were arrested in G1/S and then synchronized in G2.
Protein-Protein interaction analysis using string for upregulated genes in the G1/S phase of cdc10-129 cells using string.
Dr. Bethany Campbell and Dr. Maitreyi Das at the graduate hooding ceremony.
pombe Valentine. Septin proteins are localized in the shape of a heart in a fission yeast cell.
Crystal caves, Bermuda.
Drs. Bethany Campbell and Maitreyi Das
Das lab members past and present at Cellbio2024
WICB Panel and Networking Event: Academic Evolution: Rethinking Metrics, Rewriting Rules AT 3:15PM room 25b at Cellbio2024.
A sea gull(?) I think on the balcony of my room at the hotel in San Diego.
Das lab presentations at cellbio2024. Please see program for more details.
Me at 5 yrs old in posing traditional Indian clothes.
Celebrating Dr. Marcus Harrell! with Dr. Maitreyi Das
Dec 3rd Dhanya Kalathil - P1075 The Role of Arp2/3 complex in Regulating Mitotic Entry and progression in S. pombe [Board No. B78] Dec 4th Bethany Campbell - P1813 Modulation of Cdc42 activity regulates branched actin-mediated endocytosis during cell polarization [Board No. B83] Justin McDuffie - P2101 Cell-Cycle-Dependent Polarized Growth Inhibition in Fission Yeast [Board No. B382] Walker Vickers - P2103 Regulation of competition between multiple sites of growth in fission yeast by Rax1 and Rga3 [Board No. B384] Samridhi Pathak - P2104 Investigating the cell-cycle-dependent transition from monopolar to bipolar growth in fission yeast [Board No. B385] Marcus Harrell - P2111 Endocytosis Regulates Negative Feedback to Promote Bipolar Growth in Fission Yeast [Board No. B392]