Maitreyi Das @daslabpombe.com · Sep 6 She found that on CK666 treatment, the SPB often failed to recruit the Polo-like kinase Plo1 and the SPB docking protein Cut11. 4/
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Cut11 recruitment defects typically indicate failure to inset the SPB in the NE. Just before SPB insertion during fenestration, the SUN domain protein Sad1 forms a ring framing the fenestra. Ck666-treated cells fail to form this ring as seen with Structured illumination microscopy. 5/